Title

Ermap, a gene coding for a novel erythroid specific adhesion/receptor membrane protein.

Document Type

Article

Publication Date

2000

Keywords

Animal, Blotting-Northern, Cell-Adhesion, Cell-Adhesion-Molecules, Cell-Line, Cell-Nucleus, Chromosome-Mapping, Cloning-Molecular, Cytoplasm, DNA-Complementary, Embryo, Erythrocytes, Gene-Expression-Regulation, Gene-Expression-Regulation-Developmental, Human, In-Situ-Hybridization, K562-Cells, Luminescent-Proteins, Male, Membrane-Proteins, Mice, Mice-Inbred-C57BL, Molecular-Sequence-Data, Muridae, Recombinant-Fusion-Proteins, RNA, Sequence-Alignment, Sequence-Analysis-DNA, Sequence-Homology-Amino-Acid, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Tissue-Distribution

JAX Source

Gene 2000 Jan; 242(1-2):337-45.

Grant

HL55321/HL/NHLBI

Abstract

Ermap (erythroid membrane-associated protein), a gene coding for a novel transmembrane protein produced exclusively in erythroid cells, is described. It is mapped to murine Chromosome 4, 57 cM distal to the centromere. The initial cDNA clone was isolated from a day 9 murine embryonic erythroid cell cDNA library. The predicted peptide sequence suggests that ERMAP is a transmembrane protein with two extracellular immunoglobulin folds, as well as a highly conserved B30.2 domain and several phosphorylation consensus sequences in the cytoplasmic region. ERMAP shares a high homology throughout the entire peptide with butyrophilin, a glycoprotein essential for milk lipid droplet formation and release. A GFP-ERMAP fusion protein was localized to the plasma membrane and cytoplasmic vesicles in transiently transfected 293T cells. Northern blot analysis and in-situ hybridization demonstrated that Ermap expression was restricted to fetal and adult erythroid tissues. ERMAP is likely a novel adhesion/receptor molecule specific for erythroid cells.

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