Sex, flies and microarrays.
Document Type
Article
Publication Date
2001
First Page
355
Last Page
356
JAX Source
Nat Genet 2001 Dec; 29(4):355-6.
Abstract
In a typical microarray study, experimental RNA samples are compared to a reference sample and expression ratios are clustered. A new study of the effects of sex, aging and genotype on gene expression in Drosophila melanogaster departs from this paradigm. Highly replicated comparisons generate measures of expression that are analyzed using classical analysis of variance (ANOVA) techniques, revealing substantial differences in gene expression between the two sexes as well as smaller effects of age and strain. Moreover, this study demonstrates that replication and experimental design are essential to detecting small but biologically important differences in expression patterns.
Recommended Citation
Churchill GA,
Oliver B.
Sex, flies and microarrays. Nat Genet 2001 Dec; 29(4):355-6.