Effect of age on susceptibility to azoxymethane-induced colonic aberrant crypt foci formation in C57BL/6JNIA mice.
Document Type
Article
Publication Date
2003
Keywords
Animal, Azoxymethane, Carcinogens, Colon, Colonic-Neoplasms, Immunohistochemistry, Isoenzymes, Male, Mice, Mice-Inbred-C57BL, Prostaglandin-Endoperoxide-Synthase, SUPPORT-U-S-GOVT-NON-P-H-S, Weight-Loss
JAX Location
see Journal Stacks
JAX Source
J Gerontol A Biol Sci Med Sci 2003 May; 58(5):B400-5.
Abstract
To determine the effect of age on susceptibility to azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) formation and its underlying mechanism, young and old mice were injected with AOM weekly for 4 or 5 weeks and euthanized 5 or 6 weeks later. Given the same (12 or 15) mg/kg body weight dose of AOM, old mice had significantly more ACF than young mice. However, given the same total dose of AOM (to avoid confounding effect of higher dose to heavier old mice), at a low total dose (1.5 mg) there was no age difference, but at higher total doses (1.8 and 2.2 mg) young mice had significantly more ACF than old mice. These results indicate that the age-related susceptibility to AOM differs depending on whether administration of the carcinogen is based on weight or total dose. These age differences are not due to variations in cyclooxygenase-2 expression, cell proliferation, or AOM hydroxylase activity.
Recommended Citation
Chung H,
Wu D,
Gay R,
Han SN,
Goldin B,
Bronson R,
Mason J,
Smith DE,
Meydani SN.
Effect of age on susceptibility to azoxymethane-induced colonic aberrant crypt foci formation in C57BL/6JNIA mice. J Gerontol A Biol Sci Med Sci 2003 May; 58(5):B400-5.