Title

Slug is a novel downstream target of MyoD. Temporal profiling in muscle regeneration.

Document Type

Article

Publication Date

2002

Keywords

Gene-Expression-Profiling, Humans, Mice-Inbred-C57BL, Mice-Knockout, Muscle-Skeletal, MyoD-Protein, Promoter-Regions-Genetic, Regeneration, Transcription-Factors

JAX Source

J Biol Chem 2002 Aug; 277(33):30091-101.

Abstract

Temporal expression profiling was utilized to define transcriptional regulatory pathways in vivo in a mouse muscle regeneration model. Potential downstream targets of MyoD were identified by temporal expression, promoter data base mining, and gel shift assays; Slug and calpain 6 were identified as novel MyoD targets. Slug, a member of the snail/slug family of zinc finger transcriptional repressors critical for mesoderm/ectoderm development, was further shown to be a downstream target by using promoter/reporter constructs and demonstration of defective muscle regeneration in Slug null mice.