A comparison of cDNA, oligonucleotide, and Affymetrix GeneChip gene expression microarray platforms.
Document Type
Article
Publication Date
2004
Keywords
Animals, Comparative-Study, DNA-Complementary, Mice, Oligonucleotide-Array-Sequence-Analysis
First Page
276
Last Page
284
JAX Source
J Biomol Tech 2004 Dec; 15(4):276-84.
Abstract
We have conducted a study to compare the variability in measured gene expression levels associated with three types of microarray platforms. Total RNA samples were obtained from liver tissue of four male mice, two each from inbred strains A/J and C57BL/6J. The same four samples were assayed on Affymetrix Mouse Genome Expression Set 430 GeneChips (MOE430A and MOE430B), spotted cDNA microarrays, and spotted oligonucleotide microarrays using eight arrays of each type. Variances associated with measurement error were observed to be comparable across all microarray platforms. The MOE430A GeneChips and cDNA arrays had higher precision across technical replicates than the MOE430B GeneChips and oligonucleotide arrays. The Affymetrix platform showed the greatest range in the magnitude of expression levels followed by the oligonucleotide arrays. We observed good concordance in both estimated expression level and statistical significance of common genes between the Affymetrix MOE430A GeneChip and the oligonucleotide arrays. Despite their apparently high precision, cDNA arrays showed poor concordance with other platforms.
Recommended Citation
Woo Y,
Affourtit J,
Daigle S,
Viale A,
Johnson K,
Naggert J,
Churchill G.
A comparison of cDNA, oligonucleotide, and Affymetrix GeneChip gene expression microarray platforms. J Biomol Tech 2004 Dec; 15(4):276-84.