Title

ZFP191 is required by oligodendrocytes for CNS myelination.

Document Type

Article

Publication Date

2010

Keywords

Animals, Carrier-Proteins, Cell-Differentiation, Cell-Line, Cell-Proliferation, Cells-Cultured, Central-Nervous-System, Embryo-Mammalian, Mice-Transgenic, Mutation, Myelin-Sheath, Oligodendroglia

JAX Source

Genes Dev 2010 Feb; 24(3):301-11.

Abstract

The controlling factors that prompt mature oligodendrocytes to myelinate axons are largely undetermined. In this study, we used a forward genetics approach to identify a mutant mouse strain characterized by the absence of CNS myelin despite the presence of abundant numbers of late-stage, process-extending oligodendrocytes. Through linkage mapping and complementation testing, we identified the mutation as a single nucleotide insertion in the gene encoding zinc finger protein 191 (Zfp191), which is a widely expressed, nuclear-localized protein that belongs to a family whose members contain both DNA-binding zinc finger domains and protein-protein-interacting SCAN domains. Zfp191 mutants express an array of myelin-related genes at significantly reduced levels, and our in vitro and in vivo data indicate that mutant ZFP191 acts in a cell-autonomous fashion to disrupt oligodendrocyte function. Therefore, this study demonstrates that ZFP191 is required for the myelinating function of differentiated oligodendrocytes.