Title

MARF1 regulates essential oogenic processes in mice.

Document Type

Article

Publication Date

3-23-2012

Keywords

Amino Acid Sequence, Animals, Base Sequence, Cell Cycle Proteins, DNA Breaks, Double-Stranded, Embryonic Development, Female, Fertility, Meiosis, Mice, Molecular Sequence Data, Mutation, Oocytes, Oogenesis, Phenotype, Protein Phosphatase 2, Protein Structure, Tertiary, RNA, Messenger, Retroelements, Transcription, Genetic, Transcriptome, Up-Regulation

JAX Source

Science 2012 Mar 23; 335(6075):1496-9.

Abstract

Development of fertilization-competent oocytes depends on integrated processes controlling meiosis, cytoplasmic development, and maintenance of genomic integrity. We show that meiosis arrest female 1 (MARF1) is required for these processes in mammalian oocytes. Mutations of Marf1 cause female infertility characterized by up-regulation of a cohort of transcripts, increased retrotransposon expression, defective cytoplasmic maturation, and meiotic arrest. Up-regulation of protein phosphatase 2 catalytic subunit (PPP2CB) is key to the meiotic arrest phenotype. Moreover, Iap and Line1 retrotransposon messenger RNAs are also up-regulated, and, concomitantly, DNA double-strand breaks are elevated in mutant oocytes. Therefore MARF1, by suppressing levels of specific transcripts, is an essential regulator of important oogenic processes leading to female fertility and the development of healthy offspring.