Title

Gene expression in mouse oocytes by RNA-Seq.

Document Type

Article

Publication Date

2012

Keywords

Animals, DNA, Complementary, Female, Gene Expression Profiling, Gene Library, High-Throughput Nucleotide Sequencing, Mice, Nucleic Acid Amplification Techniques, Oocyte Retrieval, Oocytes, Quality Control, RNA, Sequence Analysis, RNA

JAX Location

Reprint Collection

JAX Source

Methods Mol Biol 2012; 825:237-51.

PMID

22144249

Abstract

Second-generation sequencers such as the Illumina GAIIx make possible the study of all transcribed loci in a genome across an almost endless dynamic range. Although typical protocols call for starting from at least 1 μg of total RNA, this is not possible when studying small tissues or rare cell types. This chapter explains how to prepare Illumina sequencing libraries from mouse oocytes. The protocol is also suitable for mural and cumulus cells, flow sorted or laser captured cells.

Please contact the Joan Staats Library for information regarding this document.

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