Title

Synapse Loss and Dendrite Remodeling in a Mouse Model of Glaucoma.

Document Type

Article

Publication Date

12-4-2015

JAX Source

PLoS One 2015 Dec 4; 10(12):e0144341.

PMID

26637126

Grant

EY021525

Abstract

It has been hypothesized that synaptic pruning precedes retinal ganglion cell degeneration in glaucoma, causing early dysfunction to retinal ganglion cells. To begin to assess this, we studied the excitatory synaptic inputs to individual ganglion cells in normal mouse retinas and in retinas with ganglion cell degeneration from glaucoma (DBA/2J), or following an optic nerve crush. Excitatory synapses were labeled by AAV2-mediated transfection of ganglion cells with PSD-95-GFP. After both insults the linear density of synaptic inputs to ganglion cells decreased. In parallel, the dendritic arbors lost complexity. We did not observe any cells that had lost dendritic synaptic input while preserving a normal or near-normal morphology. Within the temporal limits of these observations, dendritic remodeling and synapse pruning thus appear to occur near-simultaneously. PLoS One 2015 Dec 4; 10(12):e0144341.