Title

Single-cell multimodal profiling reveals cellular epigenetic heterogeneity.

Document Type

Article

Publication Date

10-2016

JAX Source

Nat Methods 2016 Oct; 13(10):833-6

PMID

27525975

Abstract

Sample heterogeneity often masks DNA methylation signatures in subpopulations of cells. Here, we present a method to genotype single cells while simultaneously interrogating gene expression and DNA methylation at multiple loci. We used this targeted multimodal approach, implemented on an automated, high-throughput microfluidic platform, to assess primary lung adenocarcinomas and human fibroblasts undergoing reprogramming by profiling epigenetic variation among cell types identified through genotyping and transcriptional analysis. Nat Methods 2016 Oct; 13(10):833-6.