Title

Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics.

Document Type

Article

Publication Date

8-22-2017

JAX Source

Cell Rep 2017 Aug 22; 20(8):1978-1990

PMID

28834758

DOI

https://doi.org/10.1016/j.celrep.2017.08.003

Abstract

There is growing interest in using embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) derivatives for tissue regeneration. However, an increased understanding of human immune responses to stem cell-derived allografts is necessary for maintaining long-term graft persistence. To model this alloimmunity, humanized mice engrafted with human hematopoietic and immune cells could prove to be useful. In this study, an in-depth analysis of graft-infiltrating human lymphocytes and splenocytes revealed that humanized mice incompletely model human immune responses toward allogeneic stem cells and their derivatives. Furthermore, using an "allogenized" mouse model, we show the feasibility of reconstituting immunodeficient mice with a functional mouse immune system and describe a key role of innate immune cells in the rejection of mouse stem cell allografts. Cell Rep 2017 Aug 22; 20(8):1978-1990