Title

Phenotyping first-generation genome editing mutants: a new standard?

Document Type

Article

Publication Date

8-2017

JAX Source

Mamm Genome 2017 Aug; 28(7-8):377-382.

PMID

28756587

DOI

https://doi.org/10.1007/s00335-017-9711-x

Abstract

The unprecedented efficiency of the CRISPR/Cas9 system in genome engineering has opened the prospect of employing mutant founders for phenotyping cohorts, thus accelerating research projects by circumventing the requirement to generate cohorts using conventional two- or three-generation crosses. However, these first-generation mutants are often genetic mosaics, with a complex and difficult to define genetic make-up. Here, we discuss the potential benefits, challenges and scientific validity of such models. Mamm Genome 2017 Aug; 28(7-8):377-382.