Title

Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA).

Document Type

Article

Publication Date

2017

JAX Location

Reprint Collection

JAX Source

Methods Mol Biol 2017; 1487:195-201.

PMID

27924568

DOI

https://doi.org/10.1007/978-1-4939-6424-6_14

Abstract

RAS/MAPK signaling responds to diverse extracellular cues and regulates a wide array of cellular processes. Given its biological importance, abnormalities in RAS/MAPK signaling cascade have been intimately implicated in numerous human diseases, including cancer. Herein, we describe a novel methodology to study activation of this pivotal signaling pathway. The Proximity Ligation Assay (PLA) is employed to monitor kinase-substrate interactions between MEK1 and HSF1, or MEK1 and ERK1 in situ. Methods Mol Biol 2017; 1487:195-201.

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