Document Type
Article
Publication Date
10-4-2019
Keywords
JGM
JAX Source
Nat Commun 2019 Oct 4; 10(1):4529
Volume
10
Issue
1
First Page
4529
Last Page
4529
ISSN
2041-1723
PMID
31586101
DOI
https://doi.org/10.1038/s41467-019-12420-1
Abstract
Dynamic alterations in the unique brain extracellular matrix (ECM) are involved in malignant brain tumors. Yet studies of brain ECM roles in tumor cell behavior have been difficult due to lack of access to the human brain. We present a tunable 3D bioengineered brain tissue platform by integrating microenvironmental cues of native brain-derived ECMs and live imaging to systematically evaluate patient-derived brain tumor responses. Using pediatric ependymoma and adult glioblastoma as examples, the 3D brain ECM-containing microenvironment with a balance of cell-cell and cell-matrix interactions supports distinctive phenotypes associated with tumor type-specific and ECM-dependent patterns in the tumor cells' transcriptomic and release profiles. Label-free metabolic imaging of the composite model structure identifies metabolically distinct sub-populations within a tumor type and captures extracellular lipid-containing droplets with potential implications in drug response. The versatile bioengineered 3D tumor tissue system sets the stage for mechanistic studies deciphering microenvironmental role in brain tumor progression.
Recommended Citation
Sood D,
Tang-Schomer M,
Pouli D,
Mizzoni C,
Raia N,
Tai A,
Arkun K,
Wu J,
Black L,
Scheffler B,
Georgakoudi I,
Steindler D,
Kaplan D.
3D extracellular matrix microenvironment in bioengineered tissue models of primary pediatric and adult brain tumors. Nat Commun 2019 Oct 4; 10(1):4529
Comments
This open access article is licensed under a Creative Commons Attribution 4.0 International License.