Document Type
Article
Publication Date
4-1-2019
Keywords
JMG
JAX Source
Life Sci Alliance 2019 Apr 1; 2(2):e201800195
Volume
2
Issue
2
ISSN
2575-1077
PMID
30936185
DOI
https://doi.org/10.26508/lsa.201800195
Abstract
The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors that foster functional maturation of immune cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation of human immunity in vivo, a species barrier between human immune cells and the mouse microenvironment limits human acquired as well as innate immune function. To study the roles of human cytokines in human acquired and innate immune cell development, we created NSG mice expressing hIL-7 and hIL-15. Although hIL-7 alone was not sufficient for supporting human NK cell development in vivo, increased frequencies of human NK cells were confirmed in multiple organs of hIL-7 and hIL-15 double knockin (hIL-7xhIL-15 KI) NSG mice engrafted with human hematopoietic stem cells. hIL-7xhIL-15 KI NSG humanized mice provide a valuable in vivo model to investigate development and function of human NK cells.
Recommended Citation
Matsuda M,
Ono R,
Iyoda T,
Endo T,
Iwasaki M,
Tomizawa-Murasawa M,
Saito Y,
Kaneko A,
Shimizu K,
Yamada D,
Ogonuki N,
Watanabe T,
Nakayama M,
Koseki Y,
Kezuka-Shiotani F,
Hasegawa T,
Yabe H,
Kato S,
Ogura A,
Shultz LD,
Ohara O,
Taniguchi M,
Koseki H,
Fujii S,
Ishikawa F.
Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice. Life Sci Alliance 2019 Apr 1; 2(2):e201800195
Comments
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