A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts.
Document Type
Article
Publication Date
12-17-2021
Publication Title
STAR Protoc
JAX Source
STAR Protoc 2021 Dec 17; 2:100866
Volume
2
Issue
4
First Page
100866
Last Page
100866
ISSN
2666-1667
PMID
34647038
DOI
https://doi.org/10.1016/j.xpro.2021.100866
Abstract
This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability. Here, a peristaltic pump and 3D-printed elements are combined to perfuse the heart with enzymes to dissociate cells. Myocytes and non-myocytes extracted using this protocol are separated by centrifugation and/or fluorescence-activated cell sorting for use in downstream applications including single-cell omics or other bio-molecular analyses. For complete details on the use and execution of this protocol, please refer to McLellan et al. (2020).
Recommended Citation
Farrugia G,
McLellan M,
Weeks K,
Matsumoto A,
Cohen C,
Krstevski C,
Gaynor T,
Parslow A,
McMullen J,
Pinto A.
A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts. STAR Protoc 2021 Dec 17; 2:100866
Comments
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