Mouse cell-mediated lympholysis assay in serum-free and mouse serum-supplemented media: culture conditions and genetic factors.


A B Peck
F H Bach

Document Type


Publication Date



Animals, Cytotoxicity Tests, Immunologic, Epitopes, Lectins, Lipopolysaccharides, Lymphocyte Activation, Lymphocytes, Mice, Mice, Inbred Strains, Polysaccharides, Bacterial, Time Factors

JAX Source

Scand J Immunol 1975; 4(1):53-62.


We describe a miniaturized cell-mediated lympholysis assay for measuring the activity of cytotoxic lymphocytes generated in mouse mixed leukocyte cultures (MLC), using either serum-free or mouse serum-supplemented media. Mixed reactions of strain combinations possessing various genetic differences at H-2 produced cytotoxic lymphocytes with cytolytic activity against cells of the donor used in the sensitization phase. Results suggest that both serologically defined and lymphocyte-defined determinants can act as effective target antigens on target cells. In contrast, no cytotoxic lymphocytes were generated against products of non-H-2 loci--M-locus, theta, or other non-H-2 antigens--that induce MLC activation.

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