Idiotypic analysis of anti-GL phi antibodies. I. Identification and strain distribution of GL-1 idiotypes.
Animals, Binding Sites, Antibody, Epitopes, Glutamates, Guinea Pigs, Immune Sera, Immunoglobulin Idiotypes, Lysine, Mice, Mice, Inbred A, Mice, Inbred AKR, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred DBA, Phenylalanine, Polymers, Rabbits, Rats
J Immunol 1980 Jun; 124(6):2870-5.
We utilized both the inhibition of antigen binding and direct idiotype binding methods to identify a new set of common idiotype determinants on anti-GL antibodies of various mouse strains. Three anti-idiotypic antisera, each prepared against individually purified B10.WB anti-GL phi antibodies, were able to detect antibody-combining site-associated common idiotypic determinants, designated GL-1 idiotype(s), in antisera with GLT-binding activity obtained from all mouse strains except strains bearing Igh-1e allotype. Anti-GL phi antisera obtained from rabbits, guinea pigs, and rats did not express detectable levels of GL-1 idiotypes. Nonresponder mice to GL phi, upon immunization with GL-F gamma G or GL phi-F gamma G produced anti-GL antibodies expressing GL-1 idiotypes. Although the magnitude of the immune response to various GL-containing polymers is controlled by distinct Ir genes, the common GL-related antigenic determinants on these polymers are able to induce anti-GL antibodies with GL-1 idiotypic specificities.