Faculty Research 1980 - 1989

Title

Generation of helper cell-independent cytotoxic T lymphocytes is dependent upon L3T4+ helper T cells.

Document Type

Article

Publication Date

1988

Keywords

Antibodies-Monoclonal, Antigens-Differentiation-T-Lymphocyte: im, Beta-2-Microglobulin: im, Cytotoxicity-Immunologic, Female, Helper-Cells: cl, im, Histocompatibility-Antigens-Class-II: im, Interleukin-2: me, Lymphocyte-Transformation, Male, Mice, Mice-Inbred-BALB-C, Mice-Inbred-C57BL, Mice-Nude, Minor-Histocompatibility-Loci, Phenotype, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes-Cytotoxic: cl, im

JAX Location

1680

Grant

AI24544-01A, 2

Abstract

The role of L3T4+ (CD4+) Th cells in generation of CTL specific for discrete minor histocompatibility Ag was investigated. Suppression of the function of Th cells in vivo by chronic treatment with anti-L3T4 mAb prevented congenic strains of mice from being primed and from generating CTL specific for Ag encoded by the minor histocompatibility loci--H-3, H-1, and B2m. Analysis of proliferative responses and lymphokine secretion of cells from animals primed with one of these minor H Ag, beta 2-microglobulin, but not treated with anti-L3T4 antibodies, indicated that L3T4- class I MHC-restricted T cells were themselves responsible for the very great majority of the observed minor H Ag-specific proliferation and secretion of lymphokines associated with both T cell proliferation and activation of CTL. All together, the data indicate that in responses against discrete minor H Ag, L3T4+Th-independent CTL are generated through an L3T4+Th-dependent pathway.

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