Faculty Research 1980 - 1989

Epidermal growth factor-induced increases in inositol trisphosphates, inositol tetrakisphosphates, and cytosolic Ca2+ in a human hepatocellular carcinoma-derived cell line.

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Chromatography-High-Pressure-Liquid, Cytosol: me, Egtazic-Acid, Epidermal-Growth-Factor-Urogastrone, Hepatoma: me, Human, Inositol-Phosphates: bi, Liver-Neoplasms: me, Sugar-Phosphates: bi, SUPPORT-U-S-GOVT-P-H-S, Tumor-Cells-Cultured

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FEBS Lett 1988 Jun 6;233(1):41-6


CA37225, CA10815, DK39808


A human hepatocellular carcinoma-derived cell line, PLC/PRF/5, was examined for its ability to respond to epidermal growth factor (EGF) exposure with increased phosphatidylinositol 4,5-bisphosphate hydrolysis. Upon addition of EGF (25 ng/ml), a rapid (10-15 s) but transient increase in Ins(1,4,5)P3 levels and large, prolonged (2 min) increases in Ins(1,3,4,5)P4 and Ins(1,3,4)P3 levels were detected. Increases in cytosolic Ca2+ were observed after a 10 to 20 s lag, reaching peak value at 1 min, and remaining elevated for 10 min. The initial burst of cytosolic Ca2+ occurred in the absence of extracellular Ca2+ and probably reflects mobilization of intracellular Ca2+ stores. In cells pretreated with EGTA, the sustained component of the Ca2+ response was not observed. Comparison of the inositol phosphate and Ca2+ responses of PLC/PRF/5 cells to responses reported in other cell types indicates that this cell line is a good model for EGF action in liver.

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