Faculty Research 1980 - 1989

Title

Control of glucose phosphorylation and glucose usage in clonal insulinoma cells.

Document Type

Article

Publication Date

1988

Keywords

Cell-Line, Clone-Cells, Comparative-Study, Glucokinase: me, Glucose: ph, me, Glycolysis, Hexokinase: me, Insulinoma: me, Islet-Cell-Tumor: me, Islets-of-Langerhans: me, Mice, Pancreatic-Neoplasms: me, Phosphorylation, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Diabetes 1988 May;37(5):563-8

Grant

DK22122, DK19525, AM33016

Abstract

Glucose metabolism was investigated in two established clonal insulinoma cell lines (RINm5F and HIT) and in a newly developed line of mouse insulinoma cells (IgSV195). The hexokinase capacity in the homogenates of RINm5F cells was 22.1 +/- 3.23 U/g protein, but glucokinase was barely detectable (0.06 +/- 0.013 U/g protein). In contrast, both HIT and IgSV195 cells contained glucokinase (1.5 +/- 0.17 and 1.0 +/- 0.16 U/g protein, respectively) in addition to hexokinase activity. Glucose usage by the intact cells qualitatively reflected the glucose phosphorylation found in the cell-free extracts. RINm5F cells exhibited a high glucose usage rate with one high-affinity component, whereas both HIT and IgSV195 cells showed two components with different glucose affinities. HIT and IgSV195 cells may be useful for a model of pancreatic beta-cell glycolysis.

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