Faculty Research 1980 - 1989
A simple technique to distinguish rat from mouse chromosomes in T cell hybridomas.
Document Type
Article
Publication Date
1988
Keywords
Chromosome-Banding, Chromosomes, Female, Hybrid-Cells, Hybridomas: ul, Mice, Rats, Rats-Inbred-Lew, Species-Specificity, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes
First Page
301
Last Page
307
JAX Source
Hybridoma 1988 Jun;7(3):301-7
Grant
CA18470, CA10815
Abstract
Antigen-specific rat mouse T-cell hybridomas, prepared by fusing the BW5147 AKR thynoma with helper T cells from rats immunized with chicken ovalbumin, express both the rat and mouse major histocompatibility complex gene products. Using these functionally defined T-cell hybridomas, we have devised a technique based on the differential staining of the heterochromatin region of the rat and mouse chromosomes to distinguish the species of origin of the chromosomes. This technique should prove useful for screening potential hybrid cell lines and should aid in differentiating mouse from rat chromosomes for gene mapping studies.
Recommended Citation
Simon D,
Valentine S,
Heber KE,
Knowles BB.
A simple technique to distinguish rat from mouse chromosomes in T cell hybridomas. Hybridoma 1988 Jun;7(3):301-7