Faculty Research 1980 - 1989

Title

Expression of hepatitis B surface antigen in human cells by a recombinant BK virus DNA vector.

Document Type

Article

Publication Date

1988

Keywords

Cell-Line, DNA-Recombinant, Genetic-Vectors, Hepatitis-B-Surface-Antigens: bi, Hepatitis-B-Virus: ge, Human, Metallothionein: ge, Polyomaviruses: ge, Promoter-Regions-(Genetics), Recombinant-Fusion-Proteins: bi, Recombinant-Proteins: bi, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Tumor-Cells-Cultured

JAX Source

J Gen Virol 1988 Feb;69 ( Pt 2):459-66

Grant

CA29797, CA3725

Abstract

The construction of the first stable human cell lines that express and secrete authentic hepatitis B virus surface antigen (HBsAg), using a BK virus (BKV) episomal plasmid vector, is described. The amount of HBsAg produced by BKV vectors (up to 600 ng/10(7) cells) was comparable to other eukaryotic vector systems. The level of HBsAg expression remained the same regardless of the orientation of the HBsAg gene, substitution of the HBsAg gene promoter with the mouse metallothionein I gene promoter or the tissue origin of the human cell lines used to establish stable cellular transformants. Northern blot analysis also indicated synthesis of normal HBsAg transcripts. Surprisingly, however, the vectors were maintained at far lower than expected copy number (one to five copies/cell). Reasons for this are discussed.

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