A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies.
Antibody-Specificity, Blotting-Southern", Cell-Line, Drug-Resistance, Horseradish-Peroxidase, Human, Hybridomas, alpha-Fetoproteins
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Mol Immunol 1988 Sep; 25(9):913-915.
A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and actinomycin D. Using this approach we produced tetradomas secreting bispecific MAbs to horseradish peroxidase and human alpha-fetoprotein.
Chervonsky, A V.; Faerman, A I.; Evdonina, L V.; Jazova, A K.; Kazarov, A R.; and Gussev, A I., " A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies." (1988). Faculty Research 1980 - 1989. 1071.