Faculty Research 1980 - 1989

Title

A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies.

Document Type

Article

Publication Date

1988

Keywords

Antibody-Specificity, Blotting-Southern", Cell-Line, Drug-Resistance, Horseradish-Peroxidase, Human, Hybridomas, alpha-Fetoproteins

JAX Location

see Journal collection

JAX Source

Mol Immunol 1988 Sep; 25(9):913-915.

Abstract

A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and actinomycin D. Using this approach we produced tetradomas secreting bispecific MAbs to horseradish peroxidase and human alpha-fetoprotein.

Please contact the Joan Staats Library for information regarding this document.

Share

COinS