A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies.

Authors

A V. Chervonsky
A I. Faerman
L V. Evdonina
A K. Jazova
A R. Kazarov
A I. Gussev

Document Type

Article

Publication Date

1988

Keywords

Antibody-Specificity, Blotting-Southern", Cell-Line, Drug-Resistance, Horseradish-Peroxidase, Human, Hybridomas, alpha-Fetoproteins

First Page

913

Last Page

915

JAX Location

see Journal collection

JAX Source

Mol Immunol 1988 Sep; 25(9):913-915.

Abstract

A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and actinomycin D. Using this approach we produced tetradomas secreting bispecific MAbs to horseradish peroxidase and human alpha-fetoprotein.

Recommended Citation

Chervonsky AV, Faerman AI, Evdonina LV, Jazova AK, Kazarov AR, Gussev AI. A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies. Mol Immunol 1988 Sep; 25(9):913-915.