Faculty Research 1980 - 1989
In developing brown adipose tissue c-myc protooncogene expression is restricted to early differentiation stages.
Document Type
Article
Publication Date
1989
Keywords
Brown-Fat: em, Cell-Differentiation, Fetal-Development, Gene-Expression, Mice, Mice-Inbred-Strains, Nucleic-Acid-Hybridization, Proto-Oncogene-Proteins-Cellular: bi, Proto-Oncogenes, RNA: ge, SUPPORT-NON-U-S-GOVT
First Page
243
Last Page
248
Abstract
The expression pattern of the c-myc protooncogene during fetal development of the mouse was studied by RNA in situ hybridization to tissue sections. In day 15.5 post coitum (p.c.) and day 16.5 p.c. embryos, distinct c-myc expression was observed at the site of brown adipose tissue development. High c-myc expression at this site coincides with expression of the NADH-dependent, cytosolic form of glycerol-3-phosphate dehydrogenase (GPDH). Fat deposition in the form of single small droplets occurs as early as day 15.5 p.c. At earlier stages (day 14.5 p.c. and earlier) the mesenchymal precursor cells at this site show neither c-myc nor GPDH expression. During fetal development the brown adipose tissue increases enormously in size. GPDH expression increases concomitantly as does multilocular fat deposition. However, c-myc expression is not detectable in brown adipose tissue later than day 16.5 p.c.
Recommended Citation
Hirning U,
Schmid P,
Schulz WA,
Kozak LP,
Hameister H.
In developing brown adipose tissue c-myc protooncogene expression is restricted to early differentiation stages. . 1989; ():243