Faculty Research 1980 - 1989

Title

Isolation and characterization of paraflagellar proteins from Trypanosoma cruzi.

Document Type

Article

Publication Date

1989

Keywords

Antibodies-Monoclonal: im, Blotting-Western, Cytoskeletal-Proteins: im, Flagella: an, Fluorescent-Antibody-Technique, Immunohistochemistry, Molecular-Weight, Proteins, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Trypanosoma-cruzi: an

JAX Source

J Biol Chem 1989 Mar 5;264(7):4071-5

Grant

AI18873

Abstract

Two different Trypanosoma cruzi polypeptides, with masses of 70 and 68 kDa were purified and characterized in this work. These two polypeptides designated PAR 1 and PAR 2, respectively, co-purified during each step of the isolation procedure and were found to be located exclusively in T. cruzi flagella by indirect immunofluorescence. A pre-embedding immunoelectron microscopy procedure, with a gold-tagged secondary antibody, permitted direct identification of PAR 2 as a component of the T. cruzi paraflagellar rod. PAR 1 and PAR 2 were found to be immunologically distinct and showed no cross-reactivity with actin, tubulin, intermediate filament proteins, or other proteins present in mammalian cells. The results presented indicate that PAR 1 and PAR 2 are the major components of T. cruzi paraflagellar filaments, and that these filaments have no counterpart in mammalian cells.

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