Faculty Research 1980 - 1989

Title

Screening for mouse retinal degenerations. I. Correlation of indirect ophthalmoscopy, electroretinograms, and histology.

Document Type

Article

Publication Date

1989

Keywords

Dark-Adaptation, Disease-Models-Animal, Electroretinography, Fixatives, Fundus-Oculi, Mice, Mice-Inbred-Strains: ge, Ophthalmoscopy, Photography, Retina: pa, Retinal-Degeneration: di, pa, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Doc Ophthalmol 1989 Mar;71(3):229-39

Grant

1R01EY0775801

Abstract

Mice with hereditary retinal degeneration have provided excellent models for human disease of the biochemical and physiological events occurring in retinal degeneration. Since a number of mouse models are available for other human conditions, more mouse retinal degenerations would be expected to be known; however, finding new models has proved difficult since the search has usually involved laborious histologic screening. We applied the clinical technique of indirect ophthalmoscopy to screen mice for retinal degeneration and then used electroretinography and histology to determine whether true retinal degeneration was present. A Dawson-Trick-Litzkow microfiber corneal electrode was used to record the electroretinogram since the fiber does not occlude the pupil in these small eyes. Normal control values were developed. As an example of the success of the technique, one strain, lethal spot (ls) on indirect ophthalmoscopy appeared to have a retinal degeneration, but these mice had a normal electroretinogram indicating a primary optic atrophy. Likewise, one ls heterozygote that was tested as a control animal and was not suspected of having a retinal degeneration had an abnormal electroretinogram and peripheral retinal degeneration.

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