Faculty Research 1980 - 1989

Title

Ly-m19: the Lyb-2 region of mouse chromosome 4 controls a new surface alloantigen.

Document Type

Article

Publication Date

1981

Keywords

Antibodies-Monoclonal: im, Antigens-Ly: ge, Chromosome-Mapping, Genes-Structural, Hybridomas: im, Linkage-(Genetics), Mice, Mice-Inbred-Strains: ge, Species-Specificity, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Immunogenetics. 1981; 13(6):539-46.

Grant

CA08748, CA25508, AI14831

Abstract

Spleen cells from an SJL mouse immunized with 70Z/3 cells, an established pre-B cell line, were fused with cells of the nonsecretory myeloma line NS.1. One established hybridoma cell line (clone K10.6) continuously secreted antibody that recognized a new antigenic specificity tentatively named Ly-m19. This newly found antigen is detectable on both T and B cells. Cytotoxicity assays reveal that 75 percent of the spleen and lymph-node cells, 35 percent of bone-marrow cells, and 15 percent of thymus cells reacted with antibody of clone K10.6. Strains expressing the specificity Ly-m19.1 are characterized by negative reactions and include the strains AKR, CE/J, RF/J, GR/A, SJL, P/J, BDP/J, and LG/J. All other strains so far tested are Ly-m19.2. This strain distribution pattern distinguishes Ly-m19 from any known murine lymphocyte alloantigen, but it parallels the Lyb-2c haplotype. Linkage test of a set of AKXL recombinant inbred strains revealed close linkage of Ly-m19 and Lyb-2 loci on mouse chromosome 4.

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