Faculty Research 1980 - 1989


The role of mRNA levels and cellular localization in controlling sn-glycerol-3-phosphate dehydrogenase expression in tissues of the mouse.

Document Type


Publication Date



Brown-Fat: en, Cerebellum: en, Cerebral-Cortex: en, Cold, Comparative-Study, Glycerolphosphate-Dehydrogenase: bi, Histocytochemistry, Immunoenzyme-Technics, Kidney: en, Liver: en, Male, Mice, Mice-Inbred-Strains, Organ-Specificity, RNA-Messenger: me, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Testis: en, Translation-Genetic

JAX Source

J-Biol-Chem. 1981 Apr 10; 256(7):3576-9.


HD06712, HD07065, GM07386


sn-Glycerol-3-phosphate dehydrogenase (glycerol-P dehydrogenase, EC activity levels in seven tissues of adult mice vary approximately 100-fold from a low of 0.1 unit/mg of protein in the cerebral cortex to a high of 8 units/mg of protein in the brown fat of cold-adapted mice; the brown fat accounts for approximately 1% of the total tissue protein. This high degree of variation in the quantitative expression of an enzyme in different tissues has provided us with a system for analyzing the mechanism by which tissue specific variation for a given gene product is controlled. To this end we have measured the amount of immunoreactive enzyme and the level of translatable glycerol-P dehydrogenase mRNA and have localized the glycerol-P dehydrogenase-positive cells by immunocytochemistry. We have found that the amount of enzyme protein in a tissue is strongly correlated with the level of its translatable mRNA (correlation coefficient = 0.996) and that in several cases the differences among tissues can be explained by differences in the numbers of glycerol-P dehydrogenase-positive cells.

Please contact the Joan Staats Library for information regarding this document.