Faculty Research 1980 - 1989

Title

Characterization of the asialoglycoprotein receptor in a continuous hepatoma line.

Document Type

Article

Publication Date

1981

Keywords

Cell-Line, Hepatoma: me, Human, Kinetics, Liver-Neoplasms: me, Orosomucoid: aa, me, Receptors-Endogenous-Substances: me, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

J Biol Chem 1981 Sep 10;256(17):8878-81

Grant

GM27989-01, CA18470, CA10815

Abstract

The asialoglycoprotein receptor has been identified on a continuous human hepatoma cell line, HepG2. This receptor requires Ca2+ for ligand binding and is specific for asialoglycoprotein. There are approximately 150,000 ligand molecules bound/cell at 4 degrees C. These receptors represent a homogeneous population of high affinity binding sites with Kd = 7 X 10(-9) M. From the rate of 125I-ASOR binding at 4 degrees C, kon was 0.95 X 10(6) M-1 min-1. Uptake of 125I-ASOR at 37 degrees C was approximately 0.02 pmol/min/10(6) cells.

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