Faculty Research 1980 - 1989

Title

Genetic polymorphism of microsomal epoxide hydrolase activity in the mouse.

Document Type

Article

Publication Date

1980

Keywords

Comparative-Study, Epoxide-Hydrolases: ge, me, Hydrogen-Ion-Concentration, Kinetics, Mice, Mice-Inbred-C57BL, Mice-Inbred-DBA, Mice-Inbred-Strains, Microsomes-Liver: en, Polymorphism-(Genetics), Recombination-Genetic, Species-Specificity, SUPPORT-U-S-GOVT-P-H-S

JAX Source

J-Biol-Chem. 1980 Sep 25; 255(18):8650-4.

Grant

R01-ES-01884, P01-CA-22484, GM18684

Abstract

Hepatic microsomal epoxide hydrolase activity (EC 3.3.2.3), assayed using styrene oxide as the substrate, has a pH optimum of 9.5 from C57BL/6J mice and a pH optimum of 8.7 from DBA/2J mice. In cross and back-cross matings between C57BL/6J and DBA/2J mice, this phenotypic difference in epoxide hydrolase activity is inherited as a single autosomal trait, with co-dominant expression in heterozygotes. Heating liver microsomes from C57Bl/6J mice at 62 degrees C for 30 min produced a slight decrease in enzyme activity, whereas the same treatment of DBA/2J microsomes reduced enzyme activity to less than 3% of its initial value. Twenty-six inbred strains of mice were examined and separated into two phenotypic classes on the basis of differences in pH optima and heat sensitivity of microsomal epoxide hyrolase activity. Eph-1 is proposed as the locus symbol of the structural gene for microsomal epoxide hydrolase, with superscripts b and d designating the alleles carried by C57BL/6J and DBA/2J mice, respectively. Using 24 recombinant inbred strains derived from C57BL/6J and DBA/2J mice, Eph-1 was found to be linked to two loci on Chromosome 1.

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