Faculty Research 1980 - 1989

Title

Response against single minor histocompatibility antigens. I. Functional and immunogenetic analysis of cloned cytolytic T cells.

Document Type

Article

Publication Date

1983

Keywords

Clone-Cells: im, Concanavalin-A, Helper-Cells: im, Interleukin-2: bi, ph, Isoantigens: im, Leukocyte-Culture-Test-Mixed, Lymphocyte-Transformation, Male, Mice, Mice-Inbred-Strains: ge, Minor-Histocompatibility-Loci, Species-Specificity, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes-Cytotoxic: im

JAX Source

J-Immunol. 1983 Nov; 131(5):2135-40.

Grant

R01, R01, R01

Abstract

A clonal approach was used to investigate the cellular basis of a T cell response to single minor histocompatibility antigens (miHA). This analysis was performed by functional and immunogenetic characterization of a large number of clones derived from short-term mixed leukocyte culture (MLC) populations generated against the miHA, H-1.3. Forty-nine clones isolated from such MLC were specifically cytolytic for H-1.3-bearing, H-2Db-compatible target cells. Thirty-seven of the 49 cytolytic clones were driven to proliferate when stimulated by spleen cells bearing the H-1.3 alloantigen in the absence of added T cell-derived growth factor(s) (GF). The remaining 12 clones proliferated only when GF was added. A strong positive correlation was observed between antigen-induced proliferation and the production of interleukin 2 (IL 2) activity. A similar correlation was observed when comparing the ability of both antigen and concanavalin A to induce IL 2 activity from the clones. These data suggest that i) antigen-driven or helper T cell-independent cytolytic T cells (HITc) are frequent components of an MLC response to a single miHA, and ii) the ability of HITc to undergo antigen-driven proliferation is related to their ability to produce antigen-induced GF.

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