Faculty Research 1980 - 1989

Title

Mouse sn-glycerol-3-phosphate dehydrogenase: molecular cloning and genetic mapping of a cDNA sequence.

Document Type

Article

Publication Date

1983

Keywords

Animal, Base-Sequence, Cloning-Molecular, DNA: ge, Glycerolphosphate-Dehydrogenase: ge, Mice, Nucleic-Acid-Hybridization, RNA-Messenger: ge, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Proc-Natl-Acad-Sci-USA. 1983 May; 80(10):3020-4.

Grant

HD06712, HD08431

Abstract

The isozymes of glycerol-3-phosphate dehydrogenase (GPDH; sn-glycerol-3-phosphate:NAD+2-oxidoreductase, EC 1.1.1.8) in tissues of the mouse are coded for by two structural genes, Gdc-1 and Gdc-2, located on chromosomes 15 and 9, respectively. In order to investigate the expression of these genes, we isolated a GPDH cDNA clone from a mRNA preparation isolated from brown adipose tissue. The GPDH cDNA clone was identified by colony hybridization and hybrid selection of a mRNA that was translated in vitro to produce immunoprecipitable GPDH protein. In blot analysis, the GPDH cDNA hybridized to a single mRNA species that migrated at the position of 23S ribosomal RNA. This GPDH cDNA clone was mapped to the Gdc-1 locus by identification of a restriction enzyme polymorphism present in genomic DNA isolated from Gdc-1 congeneic lines of mice.

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