Differentiation-defective mutants of mouse embryonal carcinoma cells: response to hexamethylenebisacetamide and retinoic acid.

Authors

P A. McCue
K I. Matthaei
M Taketo
M I. Sherman

Document Type

Article

Publication Date

1983

Keywords

Animal, Carrier-Proteins: me, Cell-Differentiation: de, Cell-Line, Mice, Mutation, Plasminogen-Activators: se, SUPPORT-U-S-GOVT-P-H-S, Teratoma: fg, me, pa, Tretinoin

First Page

416

Last Page

426

JAX Source

Dev-Biol. 1983 Apr; 96(2):416-26.

Abstract

We have generated by mutagenesis eight differentiation-defective sublines from three murine embryonal carcinoma (EC) cell lines. These mutants grossly resemble parental cells in the absence of inducers of differentiation. Based upon response to retinoic acid (RA) or hexamethylenebisacetamide (HMBA), the mutants can be grouped into three types: (a) RA-selected cells that lack cellular RA binding protein (cRABP) activity and fail to differentiate in response to RA or HMBA; (b) RA- or HMBA-selected cells that possess cRABP but differentiate poorly, if at all, in the presence of RA or HMBA; and (c) cells originally selected for lack of response to HMBA but which retain cRABP and the ability to differentiate in response to RA.

Recommended Citation

McCue PA, Matthaei KI, Taketo M, Sherman MI. Differentiation-defective mutants of mouse embryonal carcinoma cells: response to hexamethylenebisacetamide and retinoic acid. Dev-Biol. 1983 Apr; 96(2):416-26.