Faculty Research 1980 - 1989

Title

Analysis of the hematopoietic effects of new dominant spotting (W) mutations of the mouse. I. Influence upon hematopoietic stem cells.

Document Type

Article

Publication Date

1983

Keywords

Animal, Bone-Marrow: cy, tr, Colony-Forming-Units-Assay, Erythrocyte-Indices, Hematopoiesis, Hematopoietic-Stem-Cells: cy, Homozygote, Leukocyte-Count, Mice, Mice-Mutant-Strains: bl, ge, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Exp-Hematol. 1983 Jul; 11(6):452-60.

Grant

AM25305, AM27726, CA18640

Abstract

Mice carrying two mutant W alleles usually have severe macrocytic anemias which result from deficiencies of hematopoietic stem cells (CFUs) (1). Anemic W39/W39 and W41/W41 homozygotes (2) have deficiencies in the numbers of femoral stem cells which correspond to the severities of their anemias. The non-anemic W44/W44 homozygote (2) has a few stem cells as the W41/W41 mouse. Nevertheless, bone marrow implants from W44/W44 donors cure the anemias of W/Wv recipients while implants from anemic W39/W39 and W41/W41 donors do not. The peripheral hematologic differences between W41/W41 and W44/W44 homozygotes probably arise from qualitative differences intrinsic to their stem cells rather than from extrinsic hematopoietic factors. The hematopoietic environments of all three W homozygotes are relatively normal in that they support normal erythropoiesis when injected with congenic +/+ marrow. Even non-anemic W44/W44 recipients are repopulated with +/+ donor red cells, indicating that W44/W44 stem cells are at a disadvantage when competing with normal counterparts.

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