Faculty Research 1980 - 1989
Application of the ovarian teratoma mapping method in the mouse.
Document Type
Article
Publication Date
1983
Keywords
Centromere, Chromosome-Mapping, Comparative-Study, Female, Heterozygote, Isoenzymes: ge, Linkage-(Genetics), Mice: ge, Mice-Inbred-Strains, Neoplasms-Experimental: fg, Ovarian-Neoplasms: fg, Recombination-Genetic, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Teratoma: fg
First Page
797
Last Page
812
JAX Location
46,164
JAX Source
Genetics. 1983 Apr; 103(4):797-812.
Grant
GM20919, RR01183
Abstract
Murine ovarian teratomas were used to determine recombination percentages for gene-gene and centromere-gene intervals. Data were obtained utilizing a recombinant inbred strain, LTXBJ, and a number of newly developed LT/SvEi congenic strains.--Centromere-gene recombination was measured at 11.3 +/- 1.2% for the centromere of chromosome 7 - Gpi-1 interval and 15.8 +/- 2.4% for the centromere of chromosome 14 - Np-1 interval using the ovarian teratoma method. The centromere - Np-1 interval was measured at 26.5 +/- 3.6% using a standard backcross involving the Rb6Bnr Robertsonian translocation as a centromere marker.--To assess the accuracy of the ovarian teratoma mapping method, we compared the recombination frequency obtained for the Mpi-1-Mod-1 interval on chromosome 9 using the ovarian teratoma method to that obtained using a standard backcross. The recombination percentage was 22.9 +/- 5.4 using the ovarian teratoma method and 18.6 +/- 3.3 using the backcross method, indicating that the two methods produce equivalent estimates of recombination. In addition, for centromere-gene intervals known to be more than 30 cM in length, the ovarian teratoma method was consistent with classical recombination methods, yielding high recombination percentages. We conclude from these results that the ovarian teratoma mapping method is a reliable method for estimating recombination frequencies and the most accurate method available for estimating centromere-gene recombination frequency in the mouse.
Recommended Citation
Eppig JT,
Eicher EM.
Application of the ovarian teratoma mapping method in the mouse. Genetics. 1983 Apr; 103(4):797-812.