Faculty Research 1980 - 1989

Title

DNA sequence preference of the progesterone receptor.

Document Type

Article

Publication Date

1983

Keywords

Base-Sequence, Binding-Sites, Chickens, DNA: me, Female, Gene-Expression-Regulation, Genes-Structural, Ovalbumin: ge, Receptors-Progesterone: me, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Proc-Natl-Acad-Sci-USA. 1983 Jan; 80(1):16-20.

Grant

HD07857, HD07495

Abstract

Highly purified hen oviduct progesterone receptor A subunit was tested for binding to several chicken gene DNAs. Sequence preference detected by nitrocellulose filter adsorption of [32P]DNA fragments obtained from recombinant plasmids revealed a marked retention of certain DNA fragments. About a 10-fold preference was seen for DNA fragments flanking the 5' end of the steroid-regulated genes ovalbumin and gene Y. No preference was seen with analogous DNA fragments from chicken beta-globin and alpha-actin genes. Restriction endonuclease mapping suggests the presence of multiple receptor interaction sites flanking the 5' terminus of the ovalbumin gene. One of these preferential binding sites was localized between -135 and -247 base pairs upstream from the start of transcription. This region contains an 18-base-pair A + T-rich sequence, a likely candidate for the binding site itself, because earlier studies had shown receptor A to have marked preference for A + T-rich DNA.

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