Faculty Research 1980 - 1989

Title

Fetal hemoglobin production in adult baboons by 5-azacytidine or by phenylhydrazine-induced hemolysis is associated with hypomethylation of globin gene DNA.

Document Type

Article

Publication Date

1983

Keywords

Azacytidine, Fetal-Hemoglobin: ge, Gene-Expression-Regulation, Genes-Structural, Globin: ge, Hemolysis, Methylation, Papio, Phenylhydrazines, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Time-Factors

JAX Source

Prog Clin Biol Res 1983;134:489-500

Grant

HL20920-05

Abstract

Nucleoside analog were used to stimulate fetal hemoglobin synthesis in baboons. Only those nucleoside analogs (5-azacytidine and 2'-deoxy-5-azacytidine) that blocked DNA methylation caused large increases in Hb F levels. DNA extracted from bone marrow samples of these animals was cleaved with MspI or HpaII restriction enzymes and analyzed by Southern blot hybridization. Results of these experiments strongly support the hypothesis that hypomethylation of CCGG sequences in the gamma-gene region is a condition of gamma-gene expression. This also applies to Hb F elevations resulting from erythropoietic stress due to phenylhydrazine-induced hemolytic anemia.

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