
Faculty Research 1980 - 1989
Identification of a 90 000-Dalton cell surface glycoprotein with elevated expression in human hepatoma cells.
Document Type
Article
Publication Date
1983
Keywords
Cell-Line, Complement-4: an, Glycoproteins: an, im, Hepatoma: an, Human, Isoelectric-Point, Liver-Neoplasms, MembraneProteins: an, im, Molecular-Weight, Neoplasm-Proteins: an, im, SUPPORT-U-S-GOVT-P-H-S
First Page
359
Last Page
367
JAX Source
Exp Cell Res 1983 Sep;147(2):359-67
Grant
CA18470, CA10815, T32-CA09171
Abstract
We describe a human-specific cell surface glycoprotein of molecular size 90 000-dalton (90K) and isoelectric point 5 defined by a monoclonal antibody prepared using human hepatoma-mouse hepatoma hybrid cells with a limited number of human chromosomes (6, 7, 14, 20, 21, and X) as immunogens in syngeneic mice. While detectable on cultured human cells of diverse origin, expression of the 90K protein is elevated in hepatoma cells. Moreover, a protein of identical molecular size and slightly more acidic isoelectric point is present in hepatoma culture supernatant. We sought to determine the identity of the 90K protein by comparing it to two hepatoma-expressed, major histocompatibility complex-linked proteins of similar molecular size, the alpha-chain of C4 and factor B; this comparison was also prompted by the presence of human chromosome 6 in the immunizing hybrids. We find no evidence, however, for these proteins being related. Melanoma-associated antigenic determinants carried by proteins of similar molecular size have been reported, and the possible relation of these proteins to the 90K protein is discussed.
Recommended Citation
Carlin CR,
Knowles BB.
Identification of a 90 000-Dalton cell surface glycoprotein with elevated expression in human hepatoma cells. Exp Cell Res 1983 Sep;147(2):359-67