Faculty Research 1980 - 1989

Title

Demonstration that a human hepatoma cell line produces a specific insulin-like growth factor carrier protein.

Document Type

Article

Publication Date

1983

Keywords

Carrier-Proteins: me, Cell-Line, Chromatography-Gel, Hepatoma: me, Human, Insulin: me, Liver-Neoplasms: me, Molecular-Weight, Peptides: me, Somatomedins: me, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

J Clin Endocrinol Metab 1983 May;56(5):1003-8

Grant

RR01032, CA18470, CA10815

Abstract

Insulin-like growth factors (IGF's) circulate in blood complexed to specific carrier proteins. The BRL 3A and BRL 3A2 rat liver cell lines secrete a 30,000-50,000 mol wt IGF carrier protein. Since the liver parenchymal cell is a likely source of IGF carrier protein synthesis, we have evaluated medium conditioned by three human hepatocellular carcinoma- or hepatoblastoma-derived cell lines for the presence of IGF carrier protein. The HEP G2 and the HEP 3B, but not the PLC/PRF/5, cell lines secrete a specific IGF carrier protein(s) into serum-free medium. This carrier protein is specific for IGF molecules. Multiplication-stimulating activity, IGF I, and IGF II were equipotent in competing for the binding of [125I]multiplication-stimulating activity to HEP G2 medium. The HEP G2 IGF carrier protein is trypsin sensitive, acid stabile, and does not contain a glycoprotein moiety. It has a molecular size of 30,000-50,000, as assessed by Sephadex G-200 gel filtration. These studies suggest that the HEP G2 cell line is a useful model to study the synthesis and secretion of human IGF carrier protein.

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