Faculty Research 1980 - 1989

Title

Monoclonal antibody to human epidermal growth factor.

Document Type

Article

Publication Date

1983

Keywords

Antibodies-Monoclonal: im, Antibody-Specificity, Antigenic-Determinants: im, Binding-Competitive, Comparative-Study, Epidermal-Growth-Factor-Urogastrone: im, Human, Hybridomas: im, Mice, Rats, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Hybridoma 1983;2(3):321-7

Grant

GM29257, AM25436, CA18470

Abstract

A monoclonal antibody directed to a species-specific determinant of human epidermal growth factor (h-EGF) was obtained by fusing murine myeloma cells with BALB/c mouse splenocytes sensitized to h-EGF. This antibody, referred to as 863.D4, did not react with either rat or mouse epidermal growth factor or with 11 other polypeptide hormones tested as shown by solid-phase radioimmunoassay (SPRIA), and immunoprecipitation followed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). Scatchard analysis of the antibody binding to purified h-EGF revealed an apparent equilibrium dissociation constant of 1 X 10(-8) M. The antibody blocked both the binding of h-EGF and h-EGF stimulation of 3H-thymidine incorporation into DNA by greater than 90% in confluent cultures of human foreskin fibroblasts.

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