Faculty Research 1980 - 1989
Nuclear transplantation in the mouse embryo by microsurgery and cell fusion.
Document Type
Article
Publication Date
1983
Keywords
Blastocyst: ph, Cell-Fusion, Cell-Nucleus: tr, Embryo: su, Mice, Mice-Inbred-C3H, Mice-Inbred-C57BL, Mice-Inbred-ICR, Microsurgery: mt, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S
First Page
1300
Last Page
1302
JAX Source
Science 1983 Jun 17;220(4603):1300-2
Grant
CA10815, CA25875, CA27932, +
Abstract
Nuclear transplantation in the mouse embryo was achieved by using a method that combines microsurgical removal of the zygote pronuclei with the introduction of a donor nucleus by a virus-mediated cell fusion technique. Survival of embryos was greater than 90 per cent in tests of this procedure. The embryos developed to term at a frequency not significantly different from that of nonmanipulated control embryos. Because nuclei and cytoplasm from genetically distinct inbred mouse strains can be efficiently interchanged, this procedure may be useful in characterizing possible cytoplasmic contributions to the embryonic and adult phenotype.
Recommended Citation
McGrath J,
Solter D.
Nuclear transplantation in the mouse embryo by microsurgery and cell fusion. Science 1983 Jun 17;220(4603):1300-2