Faculty Research 1980 - 1989

Title

Nuclear transplantation in the mouse embryo by microsurgery and cell fusion.

Document Type

Article

Publication Date

1983

Keywords

Blastocyst: ph, Cell-Fusion, Cell-Nucleus: tr, Embryo: su, Mice, Mice-Inbred-C3H, Mice-Inbred-C57BL, Mice-Inbred-ICR, Microsurgery: mt, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Science 1983 Jun 17;220(4603):1300-2

Grant

CA10815, CA25875, CA27932, +

Abstract

Nuclear transplantation in the mouse embryo was achieved by using a method that combines microsurgical removal of the zygote pronuclei with the introduction of a donor nucleus by a virus-mediated cell fusion technique. Survival of embryos was greater than 90 per cent in tests of this procedure. The embryos developed to term at a frequency not significantly different from that of nonmanipulated control embryos. Because nuclei and cytoplasm from genetically distinct inbred mouse strains can be efficiently interchanged, this procedure may be useful in characterizing possible cytoplasmic contributions to the embryonic and adult phenotype.

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