Faculty Research 1980 - 1989

Title

A successful technique for the preservation of rabbit embryos.

Document Type

Article

Publication Date

1984

Keywords

Animals-Laboratory, Cleavage-Stage-Ovum: ph, Embryo-Transfer: ve, Female, Freezing, Morula: ph, Ovulation, Pregnancy, Preservation-Biological: mt, ve, Rabbits: ph, Superovulation, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Lab-Anim-Sci. 1984 Oct; 34(5):484-7.

Grant

RR00251

Abstract

A technique for successfully freezing, thawing and transferring rabbit embryos has been developed. Morula stage embryos were collected from super-ovulated female rabbits by flushing both oviducts and uterine horns with a tissue culture medium. Well developed, viable embryos were then transferred to freezing vials and a cryoprotectant, dimethyl sulfoxide (DMSO) was added in several steps to bring its final concentration to 1.6 molar. To freeze the embryos the temperature was lowered slowly (either 0.5 degrees C/min or 1.0 degrees C/min) to -80 degrees C at which point the vials were transferred directly to liquid nitrogen (-196 degrees C). Thawing was done at 8 degrees C/min. After thawing, phosphate buffered saline was added in a stepwise manner to dilute the DMSO. The thawed embryos were then cultured at 37 degrees C. Transfer of the embryos was accomplished by laparotomizing a pseudopregnant doe and introducing the embryos into the fimbriated ends of the oviducts. The 101 positively transferred embryos resulted in 45 implantations and 34 live born young.

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