Faculty Research 1980 - 1989

Title

A genetic analysis of natural resistance to nonsyngeneic cells: the role of H-2.

Document Type

Article

Publication Date

1984

Keywords

Comparative-Study, Crosses-Genetic, Cytotoxicity-Immunologic, H-2-Antigens: ge, im, Immunity-Natural, Killer-Cells-Natural: im, Leukemia-L1210: im, Mice, Mice-Inbred-CBA, Mice-Inbred-DBA, Mice-Inbred-Strains, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Location

88

JAX Source

Immunogenetics. 1984; 20(3):287-300.

Grant

CA28231, CA33093, GM18684

Abstract

The genetic control of natural resistance in vivo to four natural killer (NK) cell-resistant H-2 homozygous lymphoid tumor cell lines was investigated by following the survival and organ distribution of cells prelabeled with radioactive iododeoxyuridine. Backcross mice derived from DBA/2J and CBA/J parents were injected with H-2d tumor cells and tumor cell elimination was lowest in H-2d homozygotes. Natural killer cell activity was also reduced in mice with the H-2d haplotype, but no direct correlation between NK cell levels against YAC-1 or SL2-5 lymphoma cells and natural resistance in vivo was demonstrable. Analysis of 23 BXD recombinant inbred strains indicated that natural resistance to H-2d tumors was restricted to H-2b strains. There was no direct association of NK cell activity with H-2 type in the BXD strains and NK cell levels did not correlate with tumor survival in vivo. By comparing natural resistance to H-2d and H-2b tumors in DBA/2, C57BL/6, B6D2F1, and B10.D2 mice we found that H-2 nonidentity between the tumor and the host, rather than the host H-2 haplotype, determined whether natural resistance occurred. Again, NK cell activity against YAC-1 cells was not predictive of tumor survival in these strains. These results provide genetic evidence that NK cells alone cannot account for natural resistance to H-2 nonidentical cells of hemopoietic origin.

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