Faculty Research 1980 - 1989

Title

B lymphocyte precursors in embryonic and adult W anemic mice.

Document Type

Article

Publication Date

1984

Keywords

Anemia-Macrocytic: bl, im, pp, pa, Animal, B-Lymphocytes: im, pa, Bone-Marrow: pa, Cell-Count, Cell-Differentiation, Comparative-Study, Embryo: im, cy, Female, Hematopoietic-Stem-Cells: im, pa, Liver: cy, Lymphocyte-Transformation, Mice, Mice-Inbred-CBA, Mice-Mutant-Strains, Pregnancy, Spleen: pa, SUPPORT-U-S-GOVT-P-H-S

JAX Source

J-Immunol. 1984 Jun; 132(6):2724-9.

Grant

CA09190, AI20069, CA22241, +

Abstract

Mice homozygous for mutations at the dominant spotting or W locus on chromosome 5 have been extensively used as models of severe macrocytic anemia caused by defective hemopoietic stem cells. We examined cells of the developing B lineage in adult and embryonic W anemic mice both by phenotypic analyses and by three distinctly different functional assays for B lymphocyte precursors. Adult W/Wv mice had normal numbers of B cells in the spleen and bone marrow, and normal numbers of pre-B cells and cells identified by a monoclonal antibody directed to a B lineage cell surface antigen (14.8) in the bone marrow. Embryonic W/Wv and Wx/Wx mice had hypoplastic liver development at 16 days gestation with a corresponding reduction in absolute numbers of pre-B cells, 14.8+ cells, and clonable granulocyte-macrophage progenitor cells, although their frequencies were normal. As expected, spleen colony-forming units were greatly reduced both in absolute number and frequency. Adult bone marrow cells and fetal liver cells from W anemic mutants generated B cells in vitro as well as did cells from normal littermates, but W anemic cells failed to generate B lymphocytes as well in vivo. These observations likely reflect differences in precursor cells that contribute to B cell formation in these assays, and suggest that early B lineage precursors are reduced or defective in W anemic mice.

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