The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal.
Blastocyst: ph, Blood, Cells-Cultured, Comparative-Study, Embryo-Transfer, Female, Fertilization-in-Vitro, Gonadotropins-Chorionic, Male, Mice, Mice-Inbred-Strains, Morula: ph, Oocytes: de, gd, Pregnancy, Preimplantation-Phase, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-NON-P-H-S
Dev-Biol. 1984 Apr; 102(2):493-7.
The aim of this project was to compare the developmental capacities of mouse oocytes matured in vivo and in vitro. The frequencies of fertilization, preimplantation development, and birth of live offspring after transfer of morulae to uteri of pseudopregnant foster mothers were compared after germinal vesicle stage oocytes underwent spontaneous maturation in vitro, and after gonadotropin-induced maturation in vivo and ovulation. Both groups of matured ova were fertilized in vitro, and preimplantation development was carried out in vitro. Equivalent developmental capacities were observed for all comparisons between the two groups of oocytes. The acquisition of normal developmental capacity depended on the presence of serum in the oocyte maturation medium. The expansion (mucification) of the cumulus oophorus was not required for fertilization or normal development. The frequency of fertilization was lower in oocytes that matured while denuded of cumulus cells. However, when fertilization did occur in these oocytes, a normal percentage developed to live offspring. It is concluded that a normal developmental program occurs during spontaneous maturation of mouse oocytes, and that the presence of cumulus cells during spontaneous maturation may affect the oocyte's fertilizability rather than its subsequent developmental capacity.
Schroeder, A C. and Eppig, J J., " The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal." (1984). Faculty Research 1980 - 1989. 574.
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