Immunodiagnostic detection of Giardia antigen in faeces by a rapid visual enzyme-linked immunosorbent assay.

Authors

E L. Green
M A. Miles
D C. Warhurst

Document Type

Article

Publication Date

1985

Keywords

Enzyme-Linked-Immunosorbent-Assay, Feces: ps, Giardia: im, Giardiasis: dt, di, Human, Immunoenzyme-Technics, Intestinal-Diseases-Parasitic: dt, di, SUPPORT-NON-U-S-GOVT

First Page

691

Last Page

693

JAX Source

Lancet. 1985 Sep 28; 2(8457):691-3.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Giardia antigen in human faeces. 136 faecal samples were tested; 76 were positive and 27 negative for giardia cysts on microscopy, and 33 samples contained a range of other parasites. The ELISA sensitivity exceeded 98% and its specificity was 100%, by both colorimetry and direct visual interpretation. The assay could trace the decline and disappearance of antigen in faeces rendered microscopically negative by chemotherapy. This ELISA, which can be completed in less than 3 h and which does not rely on complex equipment for interpretation, should be very useful in diagnostic and epidemiological applications.

Recommended Citation

Green EL, Miles MA, Warhurst DC. Immunodiagnostic detection of Giardia antigen in faeces by a rapid visual enzyme-linked immunosorbent assay. Lancet. 1985 Sep 28; 2(8457):691-3.