Faculty Research 1980 - 1989


Remarkable homology among the internal repeats of erythroid and nonerythroid spectrin.

Document Type


Publication Date



Animal, Base-Sequence, Chickens, Cloning-Molecular, Comparative-Study, DNA: ge, Erythrocytes: ph, Evolution, Genes-Structural, Muscle-Smooth: ph, Repetitive-Sequences-Nucleic-Acid, Spectrin: ge, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

First Page


Last Page


JAX Location


JAX Source

Proc-Natl-Acad-Sci-USA. 1985 Sep; 82(17):5671-5.


HL29305, AM27726, CA2956902


A cDNA clone for nonerythroid alpha-spectrin was identified by direct immunological screening of a chicken smooth muscle cDNA library. A library prepared in the expression plasmids pUC8 and pUC9 was screened with an antiserum specific for chicken alpha-spectrin. Blots of poly(A)+ RNA from various tissues of chicken and mouse show that the cDNA hybridizes to an 8-kilobase mRNA. The cDNA hybridizes to a single-copy sequence on Southern blots of chicken genomic DNA. The complete nucleic acid sequence of the clone has a single 1419-base open reading frame. The derived amino acid sequence is organized into two partial and three complete 106-amino-acid repeats that show homology to the repeats described for human erythroid alpha- and beta-spectrin. Immunological and biochemical data indicate that chicken nonerythroid and human erythroid alpha-spectrin are two of the more widely diverged members of the spectrin family of proteins. In this respect, the degree of homology found between them was unexpected. Our data suggest a common evolutionary origin for these two alpha-spectrins and allow some predictions concerning spectrin gene structure.

Please contact the Joan Staats Library for information regarding this document.