Detection of lecithin: cholesterol acyltransferase (LCAT) in a human hepatoma cell line.
Fluorescent-Antibody-Technique, Hepatoma: en, Human, Immunodiffusion, Immunoelectrophoresis, Lecithin-Acyltransferase: an, Liver-Neoplasms: en, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
Cancer Biochem Biophys 1985 Feb;7(4):301-7
HL23719, HL23181, AG03255
A human hepatoma cell line (HepG-2) was probed for the presence of lecithin: cholesterol acyltransferase (LCAT) using an antiserum to human plasma LCAT. Double immunodiffusion analysis using antiserum to human plasma LCAT revealed a single precipitin line in the sonicated cell homogenate. This precipitin line showed a reaction of identity with highly purified plasma LCAT. The presence of LCAT within the hepatoma cells was also confirmed by an immunofluorescence test. In contrast, the cell culture supernate showed a weak and inconsistent precipitin line. These data suggest that HepG-2 cells synthesize LCAT but secretion of the enzyme by these cells into the culture medium may be partially or totally impaired.
Koren, E; McConathy, W J.; Lacko, A G.; and Knowles, B, " Detection of lecithin: cholesterol acyltransferase (LCAT) in a human hepatoma cell line." (1985). Faculty Research 1980 - 1989. 730.