Faculty Research 1980 - 1989

Title

SSEA-1, a stage-specific embryonic antigen of the mouse, is carried by the glycoprotein-bound large carbohydrate in embryonal carcinoma cells.

Document Type

Article

Publication Date

1985

Keywords

Antibodies-Monoclonal, Antigens-Neoplasm: an, Cell-Line, Electrophoresis-Polyacrylamide-Gel, Glycolipids: an, Glycoproteins, Mice, Molecular-Weight, Polysaccharides, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Teratoma: an

JAX Source

Cell Differ 1985 May;16(3):169-73

Grant

HD12487

Abstract

Molecules carrying SSEA-1 were isolated from [3H]galactose-labeled embryonal carcinoma cells by detergent solubilization followed by indirect immunoprecipitation. The antigenic molecules were degraded by extensive pronase digestion or mild alkaline treatment, and the majority of the products thus formed were so large as to be excluded from a column of Sephadex G-50. Therefore, the major carbohydrate constituent of the antigenic molecule was embryoglycan, the glycoprotein-bound large glycan in early embryonic cells. Furthermore, the binding of isolated embryoglycan with anti-SSEA-1 was directly shown by a modified Farr's assay. From these results we concluded that SSEA-1 determinant was carried by the large glycan.

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